Browsing by Author "ABDUSALAM, MOHAMED"
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thesis.listelement.badge DESIGN, SYNTHESIS AND DNA BINDING ABILITY OF NOVEL PLATINUM(II) COMPLEXES CONTAINING BIDENTATE NITROGEN AND SULPHUR DONOR LIGAND(2017-01-07) ABDUSALAM, MOHAMED; ÖZALP YAMAN, ŞenizOne of the main challenges in bioinorganic chemistry is to synthesize new DNA targeting complexes as a potential anticancer drug leads providing better efficiency with low tissue resistance compared to cisplatin. For this purpose, three novel platinum(II) complexes containing 5,6-di-2-thienyl-2,3-dihydropyrazine in asymmetric- (asy-), cis and bis- geometry have been synthesized, identified and their DNA binding ability has been studied by spectroscopic techniques and gel electrophoresis. Spectroscopic data and the calculated binding constant values were demonstrated that the synthesized drugs bind DNA in different types. While Cis and Asy complexes perform base binding covalently, bis complex interact with DNA by intercalating mode but in weaker extend. Gel electrophoresis results were in agreement with the spectroscopic findings. All the complexes induce an oxidative single strand cleavage in the presence of a reductant (ascorbic acid) on the double helix structure of DNA with the decreasing activity order from Asy to Bis. On the other hand, cytotoxicity experiments carried out at the neutral conditions, suggested that the Bis complex is the most promising anticancer agent for the treatment of Human Glioblastoma.