Browsing by Author "Albayrak, Deniz"
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Item SURFACE PATTERNING OF POLY(ε-CAPROLACTONE) SCAFFOLDS BY ELECTROSPINNING FOR CELL GUIDANCE(2022-01-21) Albayrak, Deniz; Türkoğlu Şaşmazel, HilalThe aim of this work was to produce three dimentional fibrous surface patterns of Poly(ε-Caprolactone) (PCL), onto 2D smooth solvent cast PCL surfaces with electrospinning method by using a mask/stencil for cell guidance. The characterizations of produced scaffolds were done by thickness measurements, Scanning Electron Microscopy (SEM) analyses, contact angle (CA) measurements, Fourier-transform infrared spectroscopy (FTIR) and mechanical tests. According to SEM micrographs, all of the electrospun scaffold surfaces were exhibited bead-free and uniform morphology while solvent cast surfaces were smooth and nonporous. CA measurements revealed that the solvent cast surfaces had moderate hydrophilicity (~60⁰) while electrospun regions had more hydrophobic character (~110⁰ for fully electrospun surface and ~85⁰ for electrospun patterns). Mechanical testing was showed the produced scaffolds had brittle character. Also cell culture studies were performed with mouse fibroblast (L929) cells for 7 days period and cell attachment assay, MTT assay, flourescence and SEM analyses were done. Cell culture studies were indicated that the solvent cast and electrospun parts have different characteristics for cell behaviour. Thus, it is possible to achieve cell guidance for by manupulating the cell attachment and proliferation by introducing electrospun patterns onto solvent cast surfaces.