Browsing by Author "ELMUBARK, Marwa"
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thesis.listelement.badge THE EFFECT OF FOMITOPSIS PINCOLA MUSHROOM EXTRACT ON ANTIOXIDANT ENZYME ACTIVITES(2017-01-07) ELMUBARK, Marwa; İŞGÖR, S. BelginA long time ago, mushrooms and their extracts had been widely used for medicinal and food purposes. Nutritionally, mushrooms are low in energy and fat but high in protein, carbohydrate and dietary fiber. Mushrooms are also an important source of biologically active compounds with potential medicinal value. Bioactive secondary metabolites found in mushrooms include phenolic compounds, sterols and triterpenes. In different studies with mushrooms and isolated bioactive constituents have many pharmacological effects such as anti-tumors, antioxidant, antiviral, hypocholesterolemia and hypoglycemic effects. Using of mushroom or mushroom products in our daily diet may provide health benefits. In this study, Fomitopsis pinicola mushroom extracted with seven different methods, by using three different solvents; water, methanol, and ethanol. Total phenol and flavonoid contents of all this extracts were determined to evaluate their antioxidant potency that can act as secondary defense in the biological system, beside the primary defense enzyme systems, such as catalase (CAT), superoxide dismutase, and glutathione-S- transferase. Also the effect of this mushroom on DPPH (1,1-Diphenyl-2- picrylhydrazyl) radical scavenging activity was examined. Since hot water boiled for one hour, and repeated for two times extracts has highest total phenolic contents (TPC) and total flavonoid contents (TFC) valves; 658.900 µg of GAE/ml of extract, and 245.800 µg of QE /ml of extract respectively. DPPH and all enzyme assays were continued by using this extract. This extract has ability to stabilize free radical DPPH about 70%, with IC50 value of 0.272 g/L. also this extract inhibits CAT enzyme activity as 50%, and on the other hand GST enzyme activity was inhibited by the same extract as 85%. The IC50 values of CAT and GST for this extract were calculated as 1.951×10-5 g/L, and 0.086-0.593g/L respectively. While this extract has not significant effect on SOD enzyme and IC50 value calculated as 0.001 g/L.