Graduate School of Natural and Applied Sciences
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Browsing Graduate School of Natural and Applied Sciences by Subject "Biochemistry"
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thesis.listelement.badge INVESTIGATION OF THE EFFECT OF SENESCENCE ON ANTIOXIDANT RESPONSES FOR MG-63 (HUMAN OSTEOSARCOMA) AND MC3T3-E1 (MOUSE PRE-OSTEOBLAST) CELL LINES(2023-02-01) AYAN, Fırat; İŞGÖR, Sultan BelginAging and its effects have been revealed by many researchers, the effects of aging on living systems are still being investigated today. In this study, antioxidant enzyme responses of mouse osteosarcoma (MG-63) and mouse preosteoblast cells (MC3T3- E1) against aging, which are used in many scientific studies; Superoxide Dismutase Glutathione-S-Transferase (GST), Glutathione Peroxidase (GPx) and DPPH activities were investigated. In this study before performing the enzyme assays, the protein contents of the cells were found, and they were determined as 0.05 mg/mL for MG-63 young, 0.05 mg/mL for MG-63 young, 0.05 mg/mL for MC3T3-E1 young and 0.03 mg/mL for MC3T3-E1 young were found, respectively. Enzyme activities were analyzed comparatively for passages 10, 15, 20, 25, 30, 35, 40 and 45. As can be seen, GST, GPX activities are higher in senescent cancer cells. However, SOD activity was found to be higher in aging progenitor bone cells. In DPPH activity, senescent cancer cells were found to have the highest oxidant scavenging effect with 71.2%. On the other hand, both cell lines were stained with S-A-β-Galactosidase, and β-Galactosidase accumulations due to senescence were qualitatively investigated, and blue stain accumulation was found to be higher in aged osteosarcoma cells. Finally, cell kinetics were examined, and doubling times and specific growth rates were found for the old and young passages of both cell lines used. Doubling times of cells were found to be as respectively, 4.45 hours for MG-63 young, 2.48 hours for MG-63 young, 3.04 hours for MC3T3-E1 old and 3.30 hours for MC3T3-E1 young.