article.page.titleprefix Aptamer decorated PDA@magnetic silica microparticles for bacteria purifcation
Date
2024-04-23
Journal Title
Journal ISSN
Volume Title
Publisher
Microchimica Acta
Abstract
One significant constraint in the advancement of biosensors is the signal-to-noise ratio, which is adversely affected by the presence of interfering factors such as blood in the sample matrix. In the present investigation, a specific aptamer binding was chosen for its affinity, while exhibiting no binding affinity towards non-target bacterial cells. This selective binding property was leveraged to facilitate the production of magnetic microparticles decorated with aptamers. A novel assay was developed to effectively isolate S. pneumoniae from PBS or directly from blood samples using an aptamer with an affinity constant of 72.8 nM. The capture experiments demonstrated efficiencies up to 87% and 66% are achievable for isolating spiked S. pneumoniae in 1 mL PBS and blood samples, respectively.
Description
Open Access ; Published by Microchimica Acta; https://doi.org/10.1007/s00604-024-06322-3; Murat Kavruk,Department of Medical Biology, Faculty of Medicine, İstanbul Aydın University, İstanbul 34295, Türkiye ; Zahra Babaie, Microfuidics & Lab-on-a-chip Research Group, İ.D. Bilkent University, Ankara 06800, Türkiye, UNAM-National Nanotech, Research Center and Institute Materials Science & Nanotech, İ.D. Bilkent University, Ankara 06800, Türkiye; Güneş Kibar, Microfuidics & Lab-on-a-chip Research Group, İ.D. Bilkent University, Ankara 06800, Türkiye, UNAM-National Nanotech, Research Center and Institute Materials Science & Nanotech, İ.D. Bilkent University, Ankara 06800, Türkiye, Micro Nano Particles (MNP) Research Group, Materials Science and Engineering Department, Adana Alparslan Turkes Science and Technology University, Adana 01250, Türkiye; Barbaros Çetin, Department of Medical Biology, Faculty of Medicine, İstanbul Aydın University, İstanbul 34295, Türkiye, Microfuidics & Lab-on-a-chip Research Group, İ.D. Bilkent University, Ankara 06800, Türkiye; Hasan Yeşilkaya, Yassine Amrani, Department Respiratory Sciences, University of Leicester, University Road, Leicester LE1 7RH, UK; Ali Doğan Dursun, Department of Physiology, School of Medicine, Atilim University, Ankara 06830, Türkiye; V. Cengiz Özalp, Department of Medical Biology, School of Medicine, Atilim University, Ankara 06830, Türkiye.
Keywords
Biosensor, Aptamer, Aptasensor, SELEX, Superparamagnetic, Microbeads
Citation
https://hdl.handle.net/20.500.14411/2026