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article.page.titleprefix
Targeted multidrug delivery systems to kill antibiotic-resistant Staphylococcus aureus

Abstract

Different ordered mesoporous silica (OMS) nanoparticles, ranging from regular COK-12 to COK-12 modified in terms of pore shape and size, have been employed as standard drug carriers for the controlled adsorption and release of drug molecules in comparison to well known OMS SBA-15 and MCM-41. The cytotoxicity analysis demonstrated that regular COK-12 particles were less harmful to mammalian cultured cells, causing lower apoptosis induction than modified COK-12, MCM-41, and SBA-15 particles. Thus, regular COK-12 was further used to prepare a dual antibiotic-loaded drug delivery material, followed by surface functionalization with Staphylococcus aureus-specific aptamers for targeting. The results demonstrated that the joint loading of lysozyme and vancomycin in regular COK-12 improved the ability of the antibiotic treatments to kill methicillin-resistant Staphylococcus strains via aptamer targeting. The minimum inhibitory concentration (MIC) values decreased 4.1-fold and 12-fold compared to the non-targeted use of the antimicrobial agents in homogeneous solutions for vancomycin and lysozyme, respectively, clearly demonstrating the high potential of COK-12 to be used as a carrier in multidrug therapy.

Description

Published by Journal of Drug Delivery Science and Technology, https://doi.org/10.1016/j.jddst.2023.104622, Veli Cengiz Ozalp, Department of Medical Biology, School of Medicine, Atilim University, 06830, Ankara, Turkey, Samet Ucak, Department of Medical Biology, School of Medicine, Istanbul Aydın University, 34295, Istanbul, Turkey, Ali D. Dursun, Department of Physiology, School of Medicine, Atilim University, 06830, Ankara, Turkey, Mert Sudagidan, Department of Medical Biology, School of Medicine, Atilim University, 06830, Ankara, Turkey, Oyku Icin, Cekdar Vakifahmetoglu, Department of Materials Science and Engineering, İzmir Institute of Technology, İzmir, Turkey, Laura M. Henning , Ulla Simon , Aleksander Gurlo, Technische Universität Berlin, Institute of Materials Science and Technology, Chair of Advanced Ceramic Materials, Straße des 17. Juni 135, 10623 Berlin, Germany.

Keywords

COK-12, Ordered mesoporous silica, Antimicrobials, Methicillin-resistant , taphylococcus aureus, Aptamers, Drug release

Citation

http://hdl.handle.net/20.500.14411/1847

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