article.page.titleprefix Therapeutic Efficacy of Boric Acid Treatment on Brain Tissue and Cognitive Functions in Rats with Experimental Alzheimer’s Disease
Date
2023-05-16
Journal Title
Journal ISSN
Volume Title
Publisher
Drug Design, Development and Therapy
Abstract
Introduction: Oxidative stress has an important role in the pathophysiology of Alzheimer’s disease (AD), the most common type of
dementia. Boric acid (BA) contributes significantly to the protection of the brain by reducing lipid peroxidation and supporting
antioxidant defense. We aimed to evaluate the therapeutic potential of BA treatment in AD rats.
Materials and Methods: Four groups were formed as Control (C), Alzheimer’s (A), Alzheimer’s + Boric acid (ABA), Boric acid
(BA). Intracerebroventricular injection of Streptozotocin (STZ) was preferred to create an AD. After 4 weeks, BA was applied 3 times
every other day. The Radial Arm Maze Test (RAMT) was used to evaluate memory and learning abilities. Biochemical and
histopathological evaluations were made in the hippocampus.
Results: Initial RAMT inlet/outlet (I/O) numbers were similar. Two weeks after STZ injection, I/O numbers decreased in group A and
ABA compared to group C and BA (p<0.05). After the second BA application, I/O numbers increased in the ABA group compared to
the A group (p<0.05). In group A, PON-1, TOS and OSI levels were higher and TAS levels were lower than in groups BA and C. After
BA treatment, PON-1 and OSI levels were lower in the ABA group than in the A group (p<0.05). Although there was an increase in
TAS value and a decrease in TOS, this did not make a statistical difference. The thickness of the pyramidal cell in CA1 and the
granular cell layers in the dentate gyrus, and the number of intact and degenerated neurons in the pyramidal cell layer were similar
between the groups.
Discussion: Significant improvement in learning and memory abilities after BA application is promising for AD.
Conclusion: These results show that BA application positively affects learning and memory abilities, and reduces oxidative stress.
More extensive studies are required to evaluate histopathological efficacy.
Description
Open Access, Published by Drug Design, Development and Therapy, https://doi.org/10.2147/DDDT.S405963, Çağrı Özdemir, Mamak State Hospital Department of Anesthesiology and Reanimation, Ankara, Turkey, Mustafa Arslan, Gazi University Faculty of Medicine Department of Anesthesiology and Reanimation, Ankara, Turkey, Gazi University, Life Sciences Application and Research Center, Ankara, Turkey,m Gazi University, Laboratory Animal Breeding and Experimental Research Center (GÜDAM), Ankara, Turkey, Ayşegül Küçük, Kutahya Health Sciences University Faculty of Medicine, Department of Physiology, Kutahya, Turkey, Zeynep Yığman, Gazi University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey, Gazi University Neuroscience and Neurotechnology Center of Excellence (NÖROM), Ankara, Turkey, Ali Doğan Dursun, Atılım University Faculty of Medicine Department of Physiology, Ankara, Turkey.
Keywords
Alzheimer’s disease, boric acid, cognitive function, oxidative stress
Citation
http://hdl.handle.net/20.500.14411/1932