Puigmal, Arnau CuestaAyran, MusaUlag, SongülAltan, ErayGüncü, Mehmet MücahitAksu, BurakDurukan, Kağan BerkanŞaşmazel, Hilal TürkoğluPerez, Roman A.Koç, ErkanO’Callaghan, DesmondGündüz, Oğuzhan2023-12-122023-12-122023-10-07http://hdl.handle.net/20.500.14411/18811751-6161https://doi.org/10.1016/j.jmbbm.2023.106163Published by Journal of the Mechanical Behavior of Biomedical Materials, https://doi.org/10.1016/j.jmbbm.2023.106163, Arnau Cuesta Puigmal,Roman A. Perez, Bioengineering Institute of Technology (BIT), Universitat Internacional de Catalunya (UIC), Barcelona, 08017, Spain, Musa Ayran,Songul Ulag,Eray Altan,Oguzhan Gunduz, Center for Nanotechnology & Biomaterials Application and Research (NBUAM), Marmara University, Turkey, Musa Ayran, Eray Altan, Institute of Pure and Applied Sciences, Marmara University, Department of Metallurgical and Materials Engineering, Istanbul, Turkey, Songul Ulag, Oguzhan Gunduz, Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Istanbul, Turkey, Mehmet Mucahit Guncu , Burak Aksu, Department of Medical Microbiology, Faculty of Medicine, Marmara University, Istanbul, Turkey, , Barkan Kagan Durukan, , Hilal Turkoglu Sasmazel, Department of Metallurgical and Materials Engineering, Atilim University, Ankara, Turkey, Erhan Koc, , Desmond O’Callaghan, Pharma-Assist Ltd, Unit 16, Carrigeen Business Park, Powerstown, Clonmel Co., Tipperary, Ireland.The polymeric nanofiber may interact and control certain regeneration processes at the molecular level to repair damaged tissues. This research focuses on the development of characterization and antibacterial capabilities of polyvinyl alcohol (PVA)/chitosan (CS) nanofibres containing fucoidan (FUC) for tissue engineering as a skin tissue substitute. A control group consisting of 13% PVA/(0.1)% CS nanofiber was prepared. To confer antibacterial properties to the nanofiber, 10, 20, and 30 mg of FUC were incorporated into this control group. The scanning electron microscope (SEM) proved the homogeneous and beadless structures of the nanofibers. The antibacterial activity of the 13% PVA/(0.1)% CS/(10, 20, 30) FUC was tested against the S.aureus and E.coli and the results showed that with FUC addition, the antibacterial activities of the nanofibers increased. The biocompatibility test was performed with a fibroblast cell line for 1, 3, and 7 days of incubation and the results demonstrated that FUC addition enhanced the bioactivity of the 13% PVA/(0.1)% CS nanofibers. In addition, the biocompatibility results showed that 13% PVA/(0.1)% CS/10 FUC had the highest viability value for all incubation periods compared to the others. In addition, the tensile test results showed that; the maximum tensile strength value was observed for 13% PVA/(0.1)% CS/10 FUC nanofibers.enFucoidan, Chitosan, Electrospun, Nanofibers, PVA, Skin tissue engineeringArticle