Durdabak, Dilara BuseDoğan, SonerTekol, Serap DemirÇelik, CanerÖzalp, Veli CengizTuna, Bilge Güvenç2024-04-022024-04-022024-02-08https://hdl.handle.net/20.500.14411/20102191-1363https://doi.org/10.1002/open.202300120Open Access; Published by ChemistryOpen; https://doi.org/10.1002/open.202300120; D. B. Durdabak, B. G. Tuna Department of Biophysics Faculty of Medicine Yeditepe University Istanbul 34755 (Turkey) E-mail: bilge.tuna@yeditepe.edu.tr; S. Dogan Department of Medical Biology Faculty of Medicine Yeditepe University Istanbul 34755 (Turkey); S. D. Tekol Department of Clinical Microbiology University of Health Sciences Kartal Dr. Lutfi Kirdar City Hospital Istanbul 34865 (Turkey); C. Celik Department of Emergency Medical Service Memorial Sisli Hospital Istanbul (Turkey); V. C. Ozalp Department of Medical Biology Faculty of Medicine Atilim University Ankara 06830 (Turkey).Point-of-care diagnosis is crucial to control the spreading of viral infections. Here, universal-modifiable probe-gated silica nanoparticles (SNPs) based lateral flow assay (LFA) is developed in the interest of the rapid and early detection of viral infections. The most superior advantage of the rapid assay is its utility in detecting various sides of the virus directly from the human swab samples and its adaptability to detect various types of viruses. For this purpose, a high concentration of fluorescein and rhodamine B as a reporting material was loaded into SNPs with excellent loading capacity and measured using standard curve, 4.19 μmol ⋅ g−1 and 1.23 μmol ⋅ g−1, respectively. As a model organism, severe acute respiratory syndrome coronavirus-2 (CoV-2) infections were selected by targeting its nonstructural (NSP9, NSP12) and envelope (E) genes as target sites of the virus. We showed that NSP12-gated SNPs-based LFA significantly outperformed detection of viral infection in 15 minutes from 0.73 pg ⋅ mL−1 synthetic viral solution and with a dilution of 1 : 103 of unprocessed human samples with an increasing test line intensity compared to steady state (n=12). Compared to the RT-qPCR method, the sensitivity, specificity, and accuracy of NSP12-gated SNPs were calculated as 100 %, 83 %, and 92 %, respectively. Finally, this modifiable nanoparticle system is a high-performance sensing technique that could take advantage of upcoming point-of-care testing markets for viral infection detections.enArticle