Şengel, NecmiyeKüçük, AyşegülÖzdemir, ÇağrıSezen, Şaban CemKip, GülayEr, FatmaDursun, Ali DoğanPolat, YücelKavutçu, MustafaArslan, Mustafa2024-02-142024-02-142023-11-12http://hdl.handle.net/20.500.14411/19901178-2013https://doi.org/10.2147/IJN.S432924Open Access; Published by International Journal of Nanomedicine ; https://doi.org/10.2147/IJN.S432924; Necmiye Şengel, Department of Oral and Maxillofacial Surgery, (As a Specialist in Anesthesiology and Reanimation), Gazi University Faculty of Dentistry, Ankara, Turkey; Ayşegül Küçük, Department of Physiology, Kutahya Health Sciences University Faculty of Medicine, Kutahya, Turkey; Çağrı Özdemir, Department of Anesthesiology and Reanimation, Mamak State Hospital, Ankara, Turkey; Şaban Cem Sezen, Department of Histology and Embryology, Kırıkkale University Faculty of Medicine, Kırıkkale, Turkey; Gülay Kip, Mustafa Arslan, Department of Anesthesiology and Reanimation, Gazi University Faculty of Medicine, Ankara, Turkey; Fatma Er, Department of Medical Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey; Ali Doğan Dursun, Department of Physiology, Atılım University Faculty of Medicine, Ankara, Turkey; Yücel Polat, Cardiovascular Surgery, Tekirdağ Dr. Ismail Fehmi Cumalıoğlu City Hospital, Tekirdağ, Turkey; Mustafa Kavutçu, Mustafa Arslan, Life Sciences Application and Research Center, Gazi University, Ankara, Turkey; Mustafa Arslan, Laboratory Animal Breeding and Experimental Researches Center (GÜDAM), Gazi University, Ankara, Turkey.Objective: This study aimed to demonstrate whether fullerenol C60, sevoflurane anesthesia, or a combination of both had protective effects on the liver and kidneys in lower extremity ischemia-reperfusion injury (IRI) in mice with streptozocin-induced diabetes. Methods: A total of 46 Swiss albino mice were divided into six groups as follows: control group (group C, n=7), diabetes group (group D, n=7), diabetes-ischemia/reperfusion (group DIR, n=8), diabetes-ischemia/reperfusion-fullerenol C60 (group DIR-FC60, n=8), diabetes-ischemia/reperfusion-sevoflurane (group DIR-S, n=8), and the diabetes-ischemia/reperfusion-fullerenol C60-sevoflurane (group DIR-S-FC60, n=8). Fullerenol C60 (100mg/kg) was administered intraperitoneally 30 min before the ischemia-reperfusion procedure to the fullerenol groups (DIR-FC60 and DIR-S-FC60). In the DIR groups, 2 hours (h) ischemia-2h reperfusion periods were performed. In the sevoflurane groups, sevoflurane was applied during the ischemia-reperfusion period with 100% O2. Liver and kidney tissues were removed at the end of the reperfusion procedure for biochemical and histopathological examinations. Results: In liver tissue, hydropic degeneration, sinusoidal dilatation, pycnotic nuclei, prenecrotic cells, and mononuclear cell infiltration in parenchyma were significantly more frequent in group DIR than in groups D and group C. In terms of the histopathologic criteria examined, more positive results were seen in group DIR-FC60, and when group DIR-FC60 was compared with group DIR, the difference was significant. The best results in AST, ALT, glucose, TBARS levels, and SOD enzyme activities in liver tissue were in group DIR-FC60 compared with group DIR, followed by groups DIR-S-FC60 and DIR-S, respectively. Regarding TBARS levels and SOD enzyme activities in kidney tissue, the best results were in groups DIR-FC60, DIR-S-FC60, and DIR-S, respectively. Conclusion: According to our findings, it is clear that fullerenol C60 administered intraperitoneally 30 min before ischemia, alone or together with sevoflurane, reduces oxidative stress in distant organ damage caused by lower extremity IRI, and reduces liver and kidney tissue damage in histopathologic examinations.enlower extremity, ischemia-reperfusion, diabetes, fullerenol C60, sevoflurane, kidney, liver, miceArticle