Köksal, ZeynepKurtipek, ÖmerArslan, MustafaDoğan, Dursun AliYığman, ZeynepÖzer, Abdullah2024-02-142024-02-142023-11http://hdl.handle.net/20.500.14411/19912405-8440https://doi.org/10.1016/j.heliyon.2023.e22973Open Access; Published by Heliyon; https://doi.org/10.1016/j.heliyon.2023.e22973; Zeynep Köksal, Haymana State Hospital Department of Anesthesiology and Reanimation, Ankara, Turkey; Ömer Kurtipek, Mustafa Arslan, Gazi University Faculty of Medicine Department of Anesthesiology and Reanimation, Ankara, Turkey; Mustafa Arslan, Gazi University, Life Sciences Application and Research Center, Ankara, Turkey, Gazi University, Laboratory Animal Breeding and Experimental Researches Center (GÜDAM), Ankara, Turkey; Ali Doğan Dursun, Atılım University Faculty of Medicine Department of Physiology, Ankara, Turkey; Zeynep Yığman, Gazi University Faculty of Medicine, Department of Histology and Embryology, Ankara, Turkey, Gazi University Neuroscience and Neurotechnology Center of Excellence (NÖROM), Ankara, Turkey; Abdullah Özer, Gazi University Faculty of Medicine, Department of Cardiovascular Surgery, Ankara, Turkey.Aim The aim of our study is to show whether the administration of hydrogen-rich saline solution (HRSS) intraperitoneally before left main coronary artery (LAD) ischemia protects the myocardium against ischemia-reperfusion (IR) injury. Materials and methods After ethics committee approval, 24 Wistar Albino rats were divided into 4 groups, 6 rats in each group. For experimental IR, myocardial ischemia was performed by LAD ligation. Left thoracotomy was performed without ischemia in the Control group (Group C). Left thoracotomy was performed without myocardial ischemia to the rats in the HRSS group, and HRSS was given intraperitoneally (ip) at a rate of 10 ml/kg throughout the procedure. In the MIR-HRSS group, a single dose of 10 ml/kg HRSS was administered 5 min before reperfusion. Histopathological and biochemical parameters were compared in myocardial tissue samples taken at the end of the reperfusion period. Results When the groups were compared among themselves in terms of TOS and TAS levels, there was a significant difference between the groups (p = 0.006, p = 0.002). The severity of cardiomyocyte degeneration was significantly greater in MIR group than that in the control and HRSS groups (p = 0.002 and p = 0.001, respectively), as well as severity score of cardiomyocyte degeneration was higher in MIR-HRSS group compared with HRSS group (p = 0.035). Conclusion Our study shows that HRSS is protective in IR injury, with the application of HRSS 5 min before reperfusion, interstitial edema severity, subendocardial haemorrhage are reduced, and oxidant status parameters are increased, while antioxidant status parameters are decreased. We believe that when it is supported by other studies, the protective effects of HRSS on IR damage will be shown in detail and its indications will be expanded.enHydrogen-rich saline solution; Ischemia reperfusion; Myocard; Total antioxidant status; Total oxidative statusArticle