Graduate School of Natural and Applied Sciences
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Browsing Graduate School of Natural and Applied Sciences by Author "ABDALLA, REMA A. IBRAHIM"
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thesis.listelement.badge SYNTHESIS, CHARACTERIZATION AND BINDING BEHAVIOUR OF PLATINUM(II) COMPLEX CONTAINING N- (PYRIDINE-2-YL) METHYLENE BENZAMINE WITH DNA, HSA AND BSA USING SPECTROSCOPIC TECHNIQUES(2022-02-28) ABDALLA, REMA A. IBRAHIM; ÖZALP YAMAN, Şeniz; KORKMAZ ÖZKAN, FilizBecause, the structural equivalences of cisplatin, (Pt(NH3)2Cl2, react with DNA in a similar manner with the parent drug, the new platinum based antitumor drug candidates are expected to show high antitumor activity that overcome the drug resistance and serious side effects. For this purpose, the PtCl2 complex of N-(pyridin 2-yl)methylene benzenamin ligand (L), was synthesized and characterized. The binding ability of the Pt(L)Cl2 to the calf thymus DNA and the serum albumins (BSA and HSA) were examined. In order to elucidate the mechanism of the action between the Pt(L)Cl2 complex and DNA, spectroscopic measurements were performed. The electronic absorption spectra, thermal behavior, viscosity and fluorometric titration of the Pt complex treated DNA indicated that the compound electrostatically associated with DNA. The spectroscopic and viscometric measurements also revealed that Pt(L)Cl2 interacts electrostatically with serum proteins too. The interaction is through the hydrophobic region of both proteins, where the polarity around a tryptophan residue increases and hydrophobicity of - helices increases accordingly. However no clear evidences were obtained from the detailed FTIR analysis to locate the interaction site of Pt(L)Cl2 on the protein.