Browsing by Author "Elkouha, Muna Zuam Emhemed"
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thesis.listelement.badge ANTIOXIDANT POTENTIAL ANALYSIS OF COLUMN CHROMATOGRAPHIC FRACTIONS OF EUPHORBIA PLANT EXTRACT(2022-02-25) Elkouha, Muna Zuam Emhemed; İşgör, S. BelginThe aim of this study was to assess quantity determination of Polyphenols (Total phenolic content; TPC, Total flavonoid content; TFC) and evaluate antioxidant potential of Euphorbia macroclada Boiss. leaves extract followed by their effect on antioxidant enzymes; Superoxide dismutase (SOD), Glutathione-S transferase (GST) and Catalase (CAT). The methanolic leaves extract of the E.macroclada was separated with ethyl acetate, n-hexane with a volume ratio of 1:10 into four fractions by using Column Chromatography (CC) technique. Folin-Ciocalteu’s and Aluminium chloride colorimetric method was used to analyse TPC and TFC, respectively while the antioxidant activities of column fractions were analysed by DPPH free radical scavenging assay. The inhibitory effects of E.macroclada extract were performed by testing the column fractions of extract on the activities of various antioxidant enzymes; SOD, GST, CAT. The results have shown that the TPC of the plant sample and its column fractions ranged from 43.61 to 7.26 mg/ g Gallic acid equivalent while TFC ranged from 37.05 to 2.93 mg/ g Quercetin equivalent. The DPPH IC50 values of column fractions of E.macroclada extract; FII, FIII and FIV were found to be 0.2541, 0.3409 and 3.42 g/l, respectively. The enzyme assays showed that the inhibitory effects of FII, FIII and FIV on GST enzyme activities were 92%, 98% and 78% respectively. Moreover, the inhibitory effects of both fraction; FII, FIII on CAT enzyme were about 99% while for FIV was just about 63%. Although all column fractions have strong inhibitory effects (especially FII, FIII) on GST and CAT enzyme activity, no inhibitory effects were observed on SOD.