article.page.titleprefix Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor
Date
2024-02-23
Journal Title
Journal ISSN
Volume Title
Publisher
Microchimica Acta
Abstract
This study introduces aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for adenosine triphosphate (ATP) detection where the POSS nanoparticles were synthesized in a one-step, continuous flow microfluidic reactor utilizing thermal polymerization. A microemulsion containing POSS monomers was generated in the microfluidic reactor which was designed to prevent clogging by using a continuous oil flow around the emulsion during thermal polymerization. Surfaces of POSS nanoparticles were biomimetically modified by polydopamine. The aptamer sequence for ATP was successfully attached to POSS nanoparticles. The aptamer-modified POSS nanoparticles were tested for affinity-based biosensor applications using ATP as a model molecule. The nanoparticles were able to capture ATP molecules successfully with an affinity constant of 46.5 μM. Based on this result, it was shown, for the first time, that microfluidic synthesis of POSS nanoparticles can be utilized in designing aptamer-functionalized nanosystems for biosensor applications. The integration of POSS in biosensing technologies not only exemplifies the versatility and efficacy of these nanoparticles but also marks a significant contribution to the field of biorecognition and sample preparation.
Description
Open Access; Published by Microchimica Acta ; https://doi.org/10.1007/s00604-024-06186-7; Güneş Kibar, Dept. Materials Sci. & Eng., A.T. Adana Sci. & Tech. Uni., Adana, 01250, Turkey; Güneş Kibar & Barbaros Çetin, Microfluidics & Lab-on-a-chip Research Group, İ.D. Bilkent Uni., Ankara, 06800, Turkey; Güneş Kibar, O. Berkay Şahinoğlu, Betül Kılınçlı, E. Yegan Erdem & Barbaros Çetin, UNAM-National Nanotech. Research Center and Inst. Materials Sci. & Nanotech., İ.D. Bilkent Uni., Ankara, 06800, Turkey; O. Berkay Şahinoğlu & E. Yegan Erdem, Dept. Mech. Eng., İ.D. Bilkent Uni., Ankara, 06800, Turkey; Betül Kılınçlı, Dept. Food Eng., A.T. Adana Sci. & Tech. Uni., Adana, 01250, Turkey; V. Cengiz Özalp, Dept. Medical Biology, School of Medicine, Atılım Uni., Ankara, 06836, Turkey.
Keywords
Biosensor; Polyhedral oligomeric silsesquioxane (POSS); Aptasensor; Microfluidics; ATP detection.
Citation
http://hdl.handle.net/20.500.14411/2001