article.page.titleprefix Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor
| dc.contributor.author | Kibar, Güneş | |
| dc.contributor.author | Şahinoğlu, O. Berkay | |
| dc.contributor.author | Kılınçlı, Betül | |
| dc.contributor.author | Erdem, E. Yegan | |
| dc.contributor.author | Çetin, Barbaros | |
| dc.contributor.author | Özalp, Veli Cengiz | |
| dc.date.accessioned | 2024-03-07T11:34:28Z | |
| dc.date.available | 2024-03-07T11:34:28Z | |
| dc.date.issued | 2024-02-23 | |
| dc.description | Open Access; Published by Microchimica Acta ; https://doi.org/10.1007/s00604-024-06186-7; Güneş Kibar, Dept. Materials Sci. & Eng., A.T. Adana Sci. & Tech. Uni., Adana, 01250, Turkey; Güneş Kibar & Barbaros Çetin, Microfluidics & Lab-on-a-chip Research Group, İ.D. Bilkent Uni., Ankara, 06800, Turkey; Güneş Kibar, O. Berkay Şahinoğlu, Betül Kılınçlı, E. Yegan Erdem & Barbaros Çetin, UNAM-National Nanotech. Research Center and Inst. Materials Sci. & Nanotech., İ.D. Bilkent Uni., Ankara, 06800, Turkey; O. Berkay Şahinoğlu & E. Yegan Erdem, Dept. Mech. Eng., İ.D. Bilkent Uni., Ankara, 06800, Turkey; Betül Kılınçlı, Dept. Food Eng., A.T. Adana Sci. & Tech. Uni., Adana, 01250, Turkey; V. Cengiz Özalp, Dept. Medical Biology, School of Medicine, Atılım Uni., Ankara, 06836, Turkey. | |
| dc.description.abstract | This study introduces aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for adenosine triphosphate (ATP) detection where the POSS nanoparticles were synthesized in a one-step, continuous flow microfluidic reactor utilizing thermal polymerization. A microemulsion containing POSS monomers was generated in the microfluidic reactor which was designed to prevent clogging by using a continuous oil flow around the emulsion during thermal polymerization. Surfaces of POSS nanoparticles were biomimetically modified by polydopamine. The aptamer sequence for ATP was successfully attached to POSS nanoparticles. The aptamer-modified POSS nanoparticles were tested for affinity-based biosensor applications using ATP as a model molecule. The nanoparticles were able to capture ATP molecules successfully with an affinity constant of 46.5 μM. Based on this result, it was shown, for the first time, that microfluidic synthesis of POSS nanoparticles can be utilized in designing aptamer-functionalized nanosystems for biosensor applications. The integration of POSS in biosensing technologies not only exemplifies the versatility and efficacy of these nanoparticles but also marks a significant contribution to the field of biorecognition and sample preparation. | |
| dc.description.sponsorship | The Scientific and Technological Research Council of Türkiye (TÜB˙ITAK-2218, Award Grand No.: 122C228) Adana Alparslan Türkeş Science and Technology University (Scientific Research Project, Grant No. 22103005). TÜB˙İTAK through B˙İÇABA Program. Open access funding provided by the Scientific and Technological Research Council of Türkiye (TÜB˙ITAK). | |
| dc.identifier.citation | http://hdl.handle.net/20.500.14411/2001 | |
| dc.identifier.issn | 1436-5073 | |
| dc.identifier.uri | https://doi.org/10.1007/s00604-024-06186-7 | |
| dc.language.iso | en | |
| dc.publisher | Microchimica Acta | |
| dc.relation.ispartofseries | 191; 153 | |
| dc.subject | Biosensor; Polyhedral oligomeric silsesquioxane (POSS); Aptasensor; Microfluidics; ATP detection. | |
| dc.title | Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor | |
| dc.type | Article | |
| dspace.entity.type | Article |